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KMID : 0352119950110010029
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Journal of Kyung Hee University
1995 Volume.11 No. 1 p.29 ~ p.39
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A Study on Immunoplenotypein Acute Leukemia and Lymphoproliferative Disease
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Abstract
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Immunophenotyping is a helpful tool for the diagnosis and classification of acute leukemia and lymphoproliferative diseases. To determine the diagnostic importance of immunophenotyping, 140 patients with lymphoproliferative diseases were
analyzed,
especially focused on aberrant expression of surface markers. We used the monoclonal antibodies to B cells (CD10, CD19, CD14, SmIg), T cells (CD5, CD2), myeloid antigens (CD13, CD33) and nonspecific antigen (HLA-DR) for direct or indirect
immunofluorescence method.
Myeloid antigens, CD13 and CD33 were expressed in 89% and 81% in AML respectively and 98% were positive for either one or both. Lymphoid markers were detected in 13.8% of AML, especially in FAB M4 and M5. Myeloid markers were expressed in 4/45
(8.9%) of
ALL; 2/45 (3.7%) in non-T ALL, 2/9 (22.2%) in T-ALL. CD 10 was positive in 10% (1/10) of B cell malignant lymphoma and CD5 was positive in 77.8% of CLL.
In conclusion, the diagnostic values of immunophenotyping in acute leukemia and lymphoproliferative disease are high, however, considerable number of aberrant expression were detected. Therefore combined determination by morphology and
cytochemistry is
recommended for the exact diagnosis of acute leukemia and lymphoproliferative disease.
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KEYWORD
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